Protocols, reagents and checklists for genome size estimation in plants by Flow Cytometry

Here you will find:

• Three protocols for plant genome size estimation by flow cytometry ( PDF 1) ( PDF 2) ( PDF 3)
• A checklist of the basic laboratory equipment, lab tools and consumables for plant genome size estimation by flow cytometry ( PDF 4)
• A complete description of the necessary reagents for plant genome size estimation by flow cytometry, including alternative fluorochromes and buffers ( PDF 5)
• The most commonly used standards for plant genome size estimation by flow cytometry, with a specific list for those used in Asteraceae ( PDF 6)
• A protocol for despatching plant material for genome size estimation by flow cytometry ( PDF 7)
• Information on AMERSCO DESTAINING BAGS for removing dyes from solutions ( PDF 8)

Reference books, book chapters or papers on plant genome size

Sample chapter
Doležel J, Greilhuber J, Suda J. 2007. Flow Cytometry with Plant Cells Analysis of Genes, Chromosomes and Genomes. Weinheim: Wiley-VCH Verlag GmbH & Co. KGaA. Gregory TR (ed.). 2005. The evolution of the genome. San Diego: Elsevier Academic Press. Greilhuber J, Doležel J, Wendel J, Leitch IJ. 2012. Plant Genome Diversity Volume 2. Physical Structure, Behaviour and Evolution of Plant Genomes. Vienna: Springer Verlag GmbH. Catrice O, Coba de la Peña T, Brown SC. 2006. Applications en biologie végétale : contraintes, succès, espoirs. In: “La cytométrie en flux”. Ronot X, Grunwald D, Mayol JF, Boutonnat J, (eds). Paris: Tec & Doc – Lavoisier, chpt. 12: 235–253 Doležel J, Greilhuber J, Suda J. 2007. Estimation of nuclear DNA content in plants using flow cytometry. Nature Protocols, 2: 2233–2244. Marie D, Brown SC. 1993. A cytometric exercise in plant DNA histograms, with 2C values for 70 species. Biology of the Cell, 78: 41–51.