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Protocols, reagents and checklists for genome size estimation in plants by Flow Cytometry

Here you will find:


We wish to thank Prof. Sonja Siljak-Yakovlev (Université Paris Sud – CNRS), Dr. Spencer C. Brown, Dr. Ilia Leitch, Prof. Mike Bennett (Jodrell Laboratory, Royal Botanical Gardens, Kew) and Prof. Johann Greilhuber for sharing their knowledge, and experience on the technique.


Reference books, book chapters or papers on plant genome size

Sample chapter
Doležel J, Greilhuber J, Suda J. 2007. Flow Cytometry with Plant Cells Analysis of Genes, Chromosomes and Genomes. Weinheim: Wiley-VCH Verlag GmbH & Co. KGaA.

Gregory TR (ed.). 2005. The evolution of the genome. San Diego: Elsevier Academic Press.

Greilhuber J, Doležel J, Wendel J, Leitch IJ. 2012. Plant Genome Diversity Volume 2. Physical Structure, Behaviour and Evolution of Plant Genomes. Vienna: Springer Verlag GmbH.

Catrice O, Coba de la Peña T, Brown SC. 2006. Applications en biologie végétale : contraintes, succès, espoirs. In: “La cytométrie en flux”. Ronot X, Grunwald D, Mayol JF, Boutonnat J, (eds). Paris: Tec & Doc – Lavoisier, chpt. 12: 235–253

PDF Doležel J, Greilhuber J, Suda J. 2007. Estimation of nuclear DNA content in plants using flow cytometry. Nature Protocols, 2: 2233–2244.

Marie D, Brown SC. 1993. A cytometric exercise in plant DNA histograms, with 2C values for 70 species. Biology of the Cell, 78: 41–51.